APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human FTH1 protein, and it does not cross react with human FTMT, FTHL17, and FTL protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Ferritin Heavy Chain.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ferritin heavy chain 1 , FHC , FTH , FTHL6 , HFE5 , PIG15 , PLIF
Cellular Localization
Cytoplasm
Background
This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX637654 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 15% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (GTX637654) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637654 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (GTX637654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637654 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (GTX637654) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637654 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (GTX637654) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
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REVIEW
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